Neurons in Serial Sections—4: Continue Tracing
You've moved to the subsequent section and aligned tissue and tracing from the first section.
Continue tracing the neuronal processes in the current section:
- Move to any of the endings adjacent to traced endings identified in the previous section using Joy Track or the Go To function in Macro View.
- Live images: Switch to Joy Free mode and focus on the process ending then exit the Joy Free mode.
- Acquired images: Focus on the process ending using PageUp/PageDown or the mouse wheel.
- Optional: If you are manually setting the Z value, use Move>Set Current Z to set the Z value to the value expected in the Serial Section Manager.
- Connect the endings from the previous section to the processes in the current section:
- Click Edit>Select Objects.
- Hover over the ending.
- Right-click and select Add to Ending.
- Click Yes to "continuing in a new section." Neurolucida exits the Edit mode and enters the Tracing mode; a flashing cursor appears over the ending.
- Continue tracing the process, including branch points and endings. See Tracing Trees in Single Sections (2D).
- If you've synchronized the Z meter to the Serial Section Manager (step 4), the tracings should be properly aligned with the contours of the anatomical areas.
- When you finish one process, move to another ending identified in the previous section and repeat steps 2-6.
- Once all of the processes have been traced in the current section, use Move>Meander Scan to scan the region of interest for any branches that may have been missed.
- You may also use the Go To function in Macro View or the Where Is window.
- Move to the next section and repeat aligning and tracing.
Optional: Splice process fragments 